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Code : 19300-2103      Created Date : Monday, September 25, 2017   Update Date : Monday, October 2, 2017    Visit : 1655

Dr. Rahau Shirazi gave a lecture on “New Lipid-based Materials with Gene Delivery and Biomaterials Applications” at the faculty of pharmacy

Dr. Rahau Shirazi gave a lecture in the title of "New Lipid-based Materials with Gene Delivery and Biomaterials Applications " on Sunday, Sep 24th at school of pharmacy.

 

The Summary of the presentation:

 

Environmentally responsive materials, i.e., materials that are designed to react to changes in their environment with a modification in their properties or structure, draw growing interests especially for synthetic materials for gene therapy and biomaterials applications. Environmentally responsive lipid materials are designed to be responsive to (i) reducing milieu of the cytoplasm and (ii) low pH of late endosome. (i) The cleavable multivalent lipids (CMVLs) are designed with a disulfide bond in the linker between cationic headgroup and hydrophobic tails. CMVLs are designed to show the effect of lipid-nucleic acid complexes degradation in cytoplasm on nucleic acid delivery and cytotoxicity, (ii) an acid-labile PEG-lipid (HPEG۲K-lipid) capable of losing PEG chains at the pH of late endosomes is tailored for optimum circulation lifetime of liposomes as well as efficient endosomal release. Here, the efficient synthesis is presented as well as extensive characterization of the series of tailored cleavable multivalent lipids (CMVLn, with n = ۲ to ۵ the number of positive headgroup charges at full protonation). The self-assembled complexes of the CMVLs and DNA are a prototypical environmentally responsive material, undergoing extensive structural rearrangement when exposed to reducing agents. CMVL–DNA complexes are employed as carriers for nucleic acids in DNA transfection as well as small interfering RNA silencing experiments. The employment of CMVLs in complexes with nucleic acids presents the effect of CMVLs degradation on cytotoxicity, DNA transfection as well as siRNA silencing efficiency. The investigation of structural evolution of CMVL–DNA complexes at varied complex composition, temperature and incubation time was assessed employing small-angle X-ray scattering (SAXS) and wide-angle X-ray scattering (WAXS).

 

Here below please find her presentation:

 

 

 

 

 

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Pooja Bhatkar  | Sunday, January 14, 2018
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Phospholipids for liposomes available from VAV Life Sciences, India.
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